Microarraynalysis Results

In this experiment we found that there were two genes only turned on in cancerous cells(1 and 5) and two genes on in healthy cells (3 and 6). One of the other spots held a gene that was not turned on in either cell(4). The other was colored purple(2) and therefore on in both the cancerous and healthy tissues. The 4 differences of color between the two healthy blue genes and the red cancerous genes shows that the cancer cell is missing too necessary genes that probably kill unhealthy cells, and have two different cells turned on that probably send signals for the cancerous cell to continue to multiply. Sources of error could conclude from the short amount of time number 2 stayed purple or from the dye not being in high enough concentration. In conclusion this lab went very well.

Microarraynalysis Healthy and Cancerous Genes

In microarray analysis genes of comparing factors such as cancerous and healthy DNA are put onto DNA chip. The DNA chip would be used to analyze complementary DNA made from mRNA isolaed from cancerous and noncancerous tissue. The samples are flagged with dyes and the extent to which a flagged gene adheres to its complement reveals which genes show up in cancerous tissues as well as noncancerous tissues. This information is all processed by a computer and saves a lot of money and time. A single microarray can have more than 30,000 spots of DNA, each being a different gene. Some genes are not transcribed to RNA and eventually protein in cancerous cells and some are made too much of in cancerous cells. Finding the exact genes can help medical personal to create drugs to directly treat and eliminate cancer. We are personally using 6 genes in this lab for our microarray.

CSI Acalanes Results and Discussions.

Through electrophoresis, Katie Records DNA was matched to the DNA found upon the body of poor Trevor. The patterns in the DNA were exactly the only for Katie's DNA. The lab was really fun to carry through. Placing the DNA into the gel took a manner of precision and it really felt like we were practicing biotechnology for a living. The different coloring added to the long and short strands made it easy to identify. It was interesting that almost all the DNA samples had similar strand sections except for different strand. I believe some possible sources of error could be the partial breaking down of the DNA samples due to waiting overnight for the next class period. Also our table had the positive and negative ends mixed up and although I think we did it the right way it could be possible that we screwed up. Overall this was a very successful lab and I feel I learned a lot.