In this lab we will perform genetic transformation. Genetically Modified crops are made this way, insulin is created in bacteria for diabetes, and to digest oil spills etc. In this experiment we will be transform bacteria with a gene coding for Green Fluorescent Protein. Therefore the bacteria will glow green under ultraviolet light. In this experiment we will be transferring the gene from one organism to another with the help of a plasmid( contains DNA that bacteria keeps when it needs to adapt quickly.) The pGLO plasmid contains penicillin resistance as well as the GFP, as well as a gene regulation system to control expression of the protein in transformed cells.
In this lab we will have three main steps to move the plasmid through the ecoli cell membrane
1.Usae a transformation solution of CaCl2.
2. Carry out heat shock.
growing the cells in presence of penicillin
3.Provide the cells with nutrients and an incubation period to allow them to express their new genes.
