I was actually absent for this lab but the procedure was pretty similar to most of our other labs. In this case samples of different fish, were cut off placed with a solution of buffer, incubated for a brief period and then heated up to denature the protein. Next gel electrophoresis is run on all of the proteins including, lanes set aside in order to prove or disprove if the first step worked. In our experiment the different lanes of the proteins all showed up except for that of the fly. Unfortunately enough flies have very little muscle tissue and in order to get enough tissue we needed about fifty flies rather than 3 or 4. From our results we were able to determine that the tuna and salmon were most closely related, followed by the shrimp and than the scallop, and than the fake crab, crab, and the fly didnt show up. Some sources of error could be not extracting enough protein, gaining some protein from particles floating in the air, mistaking certain bands because they were soo close together and etc.
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